{"id":2323,"date":"2015-07-17T14:17:23","date_gmt":"2015-07-17T14:17:23","guid":{"rendered":"http:\/\/www.richardmudhar.com\/?p=2323"},"modified":"2018-01-04T18:41:45","modified_gmt":"2018-01-04T18:41:45","slug":"using-the-microscope-to-grade-compost-and-compost-extract","status":"publish","type":"post","link":"https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/","title":{"rendered":"Using the microscope to grade compost and compost extract"},"content":{"rendered":"<p>This is using Elaine Ingham&#8217;s microscopy techniques to investigate thermal compost &#8211; some of what I saw. I am at an early stage of being able to do this, so any errors are mine and not Elaine Ingham&#8217;s \ud83d\ude09 The principle is to classify organisms by their morphology &#8211; aerobic fungi tend to have a colour, diameter wider than 2.5\u00b5m and\/or have uniform septa. Spiral structures are bad, indiciative of anaerobic conditions, and ciliates (hairs all over the body) also indicate anaerobic &#8211; bad- conditions. Apart form the spirochete most of these are good.<\/p>\n<div id='gallery-1' class='gallery galleryid-2323 gallery-columns-3 gallery-size-thumbnail'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_fdscn2717\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2717.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2324\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2717.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2717.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2717.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2324'>\n\t\t\t\tfungal hypha with septum 400x\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_fdscn2713\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2713.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2325\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2713.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2713.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2713.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2325'>\n\t\t\t\tfungal hypha 250x\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_fdscn2709\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2709.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2326\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2709.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2709.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2709.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2326'>\n\t\t\t\tfungal hypha 400x\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_sdscn2705\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_sDSCN2705.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2327\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_sDSCN2705.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_sDSCN2705.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_sDSCN2705.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2327'>\n\t\t\t\tspirochete &#8211; bad sort of bacteria. 250x\n\nI saw only one and this one inactive!\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_pdscn2702\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_pDSCN2702.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2328\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_pDSCN2702.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_pDSCN2702.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_pDSCN2702.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2328'>\n\t\t\t\tmisc protozoa 250x\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_fdscn2701\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2701.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2329\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2701.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2701.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2701.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2329'>\n\t\t\t\tfungal hypha 400x\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/1507_fdscn2695\/'><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2695.jpg?resize=150%2C150&amp;ssl=1\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-2330\" srcset=\"https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2695.jpg?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2695.jpg?zoom=2&amp;resize=150%2C150&amp;ssl=1 300w, https:\/\/i0.wp.com\/www.richardmudhar.com\/blog\/wp-content\/uploads\/2015\/07\/1507_fDSCN2695.jpg?zoom=3&amp;resize=150%2C150&amp;ssl=1 450w\" sizes=\"auto, (max-width: 150px) 85vw, 150px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-2330'>\n\t\t\t\tfungal hypha 250x\n\t\t\t\t<\/figcaption><\/figure>\n\t\t<\/div>\n\n<p>This is on a 5x dilution, the recommended intial conditions (use 1ml of compost and make up to 5ml total with water left to stand so the chlorine has gone).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This is using Elaine Ingham&#8217;s microscopy techniques to investigate thermal compost &#8211; some of what I saw. I am at an early stage of being able to do this, so any errors are mine and not Elaine Ingham&#8217;s \ud83d\ude09 The principle is to classify organisms by their morphology &#8211; aerobic fungi tend to have a &hellip; <a href=\"https:\/\/www.richardmudhar.com\/blog\/2015\/07\/using-the-microscope-to-grade-compost-and-compost-extract\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Using the microscope to grade compost and compost extract&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[275,14],"tags":[353,306,308],"class_list":["post-2323","post","type-post","status-publish","format-standard","hentry","category-greenmantle","category-smallholding","tag-353","tag-compost","tag-ingham"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p5aOO7-Bt","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/posts\/2323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/comments?post=2323"}],"version-history":[{"count":2,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/posts\/2323\/revisions"}],"predecessor-version":[{"id":3200,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/posts\/2323\/revisions\/3200"}],"wp:attachment":[{"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/media?parent=2323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/categories?post=2323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.richardmudhar.com\/blog\/wp-json\/wp\/v2\/tags?post=2323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}